白藜芦醇对氧糖剥夺过程中 H9C2 细胞的保护机制

The protective mechanism of resveratrol on H9C2 cells under oxygen-glucose deprivation

摘要:

〔摘 要〕 目的：白藜芦醇是一种具有生物活性的多酚类物质，生物学功能目前尚不清楚。本研究旨在探讨白藜芦醇对 氧糖剥夺（OGD）过程中心肌细胞损伤的保护机制。方法：采用大鼠心肌细胞 H9C2 细胞，建立 OGD 模型；经白藜芦醇干 预后，酶联免疫吸附试验（ELISA）检测超氧化物歧化酶（SOD）、丙二醛（MDA）的表达量，细胞活性检测试剂盒（CCK8） 检测细胞活性，Westernbolt、免疫荧光检测铁死亡相关蛋白的变化情况。结果：白藜芦醇能增强 OGD 诱导 H9C2 细胞中 SOD 的表达水平，降低 MDA 的表达，增强细胞活性，差异具有统计学意义（P ＜ 0.05）；同时白藜芦醇也能够显著提高 铁死亡相关蛋白谷胱甘肽过氧化物酶 4（GPX4）和铁蛋白重链 1（FTH1）的表达，差异具有统计学意义（P ＜ 0.05）。结论： 白藜芦醇通过降低氧化应激水平和减轻铁死亡来保护 OGD 条件下心肌细胞损伤。白藜芦醇可以作为一种预防心肌缺血 / 再 灌注（I/R）损伤的潜在药物。

Abstract:

〔Abstract〕 Objective Resveratrol is a kind of polyphenols with biological activity, but its biological function is still unclear. This study aimed to investigate the protective effect of Res on myocardial I/R injury and to explore its potential mechanism. Methods H9c2 cells were used for the in vitro experiments and oxygen-glucose deprivation/reoxygenation (OGD/R) model was established.After resveratrol treatment, the expression levels of SOD and MDA were detected by ELISA, the cell activity was detected by CCK8, and the changes of iron death related proteins were detected by Western-bolt and immunofl uorescence. Results Resveratrol could enhance the activity and decrease oxidative stress (P < 0.05); Meanwhile, the expression of glutathione peroxidase 4 (GPX4) and ferritin heavy chain 1 (FTH1) were signifi cantly increased (P < 0.05) Conclusions Resveratrol protects against myocardial I/R injury via reducing oxidative stress and attenuating ferroptosis. Resveratrol could be a potential agent to the prevention of myocardial I/R injury.