lncRNA MALAT1 在妊娠糖尿病孕妇 血清中的表达水平及临床意义

Expression and Clinical Significance of lncRNA and MALAT1 in Serum of Pregnant Women with Gestational Diabetes Mellitus

摘要:

〔摘 要〕 目的：探究长链非编码核糖核酸（lncRNA）肺腺癌转移相关转录本 1（MALAT1）在妊娠糖尿病孕妇血清中 的表达水平及临床意义。 方法：在 2017 年 1 月至 2020 年 1 月间抽选深圳市第二人民医院收治的 200 例妊娠糖尿病患者作 为研究对象，归为观察组（包括重度妊娠糖尿病者和轻度妊娠糖尿病者）；同期选择在本院行产检的 100 例健康孕妇作为 对照组，均给予血清 lncRNA MALAT1 水平、空腹血糖检测。比较观察组、对照组孕妇的 lncRNA MALAT1 水平、空腹血 糖和不良妊娠结局发生率；比较轻度妊娠糖尿病者、重度妊娠糖尿病者的不良妊娠结局发生率；另根据观察组血清 lncRNA MALAT1 水平检测结果分为 lncRNA MALAT1 低表达组、lncRNA MALAT1 高表达组，比较两组孕妇的不良妊娠结局发生率， 分析疾病严重程度、不良妊娠结局与血清 lncRNA MALAT1 水平之间的关系。 结果：观察组的 lncRNA MALAT1 水平和空 腹血糖高于对照组；观察组的不良妊娠结局发生率为 31.50 %，高于对照组的 14.00 %；重度妊娠糖尿病者的不良妊娠结局 发生率为 51.47 %，高于轻度妊娠糖尿病者的 21.21 %；lncRNA MALAT1 高表达组的不良妊娠结局发生率为 39.45 %，高于 lncRNA MALAT1 低表达组的 21.98 %；差异均具有统计学意义（P ＜ 0.05）。 结论：妊娠糖尿病患者的 lncRNA MALAT1 水平呈高表达状态，且水平越高，患者的空腹血糖和不良妊娠结局发生率越高，病情越严重。

Abstract:

〔Abstract〕 Objective To explore the expression level and clinical significance of long strands of non-coding RNA(lncRNA) and metastasis–associated lung adenocarcinoma transcript 1 (MALAT1) in pregnant women with gestational diabetes mellitus. Methods 200 patients with gestational diabetes admitted to Shenzhen Second People's Hospital from January 2017 to January 2020 were selected as the study group. (including pregnant women with severe gestational diabetes mellitus and mild gestational diabetes); 100 healthy pregnant women in our hospital were selected as control group. Serum lncRNA, MALAT1 levels and fasting blood glucose were tested. The levels of lncRNA, MALAT1, fasting blood glucose and adverse pregnancy outcomes were compared between the observation group and the control group. In addition, according to the detection results of serum lncRNA and MALAT1 levels in the observation group, they were divided into lncRNA, MALAT1 low expression group, lncRNA and MALAT1 high expression group. The incidence of adverse pregnancy outcomes in the two groups was compared, and the relationship between disease severity, adverse pregnancy outcomes and serum lncRNA and MALAT1 levels was analyzed. Results The levels of lncRNA, MALAT1 and fasting blood glucose in the observation group were higher than those in the control group; The incidence of adverse pregnancy outcomes in the observation group was 31.50%, higher than 14.00% in the control group; The incidence of adverse pregnancy outcomes in severe gestational diabetes mellitus was 51.47%, higher than 21.21% in mild gestational diabetes mellitus. The incidence of adverse pregnancy outcomes in lncRNA and MALAT1 high expression group was 39.45%, which was higher than 21.98% in lncRNA and MALAT1 low expression group; The differences were statistically significant (P < 0.05). Conclusion The levels of lncRNA and MALAT1 in patients with gestational diabetes mellitus are highly expressed. The higher the level, the higher the incidence of fasting blood glucose and adverse pregnancy outcomes, the more serious the disease is.