Abstract:〔Abstract〕 Objective To explore the efficacy and safety of 3D printing navigation template in the operation of lower cervical spine fracture and dislocation. Methods A total of 76 patients with lower cervical spine fractures and dislocations admitted to the First Affiliated Hospital of Henan University from January 2017 to January 2019 were selected as the research objects. All patients were computed tomography (CT) scan in a row, will take the image data, the import to the 3D model, to STL is derived, and the 3D reconstruction mode, the pedicle screw and template matching. After surgery, the screw position was assessed under the guidance of imaging techniques and complications were counted. Results In 76 patients, 360 screws were inserted after 3D printed navigation templates were used. After surgery, X-ray film results showed that cervical vertebra fracture reduction is good, good physiological curvature. Postoperative CT examination showed that 360 pedicle screws were located in the pedicle, and the navigation template closely fitted the exposed posterior neck structure, showing good stability. There was no compression of blood vessels, spinal cord, nerves, etc. One year postoperatively, X-ray examination showed good union without loosening or nailing. All screws were successfully inserted without complications. Conclusion In the treatment of fracture and dislocation of the lower cervical spine, the application of 3D printed navigation template can improve the accuracy of screw placement, without complications and with high safety