Abstract:〔Abstract〕 Objective To investigate the clinical effect of single-port laparoscopic high ligation of the vaginal process in the treatment of hydrocele in children. Methods A total of 260 children with hydrocele treated in Fuzhou Children's Hospital from March 2016 to March 2021 were selected. The children were divided into laparoscopic group and traditional group by random number table method, with 130 cases in each group. The laparoscopic group was treated with single-port laparoscopic high ligation of vaginal process, while the traditional group was treated with conventional open surgery. Perioperative indicators, incision healing, children's behavioral pain scale score, complications, and inflammation levels before and after surgery at 1 and 3 days were compared between the two groups. Results The incision length, operation time and hospital stay in the laparoscopic group were shorter than those in the traditional group, the amount of intraoperative blood loss was less than that in the traditional group, and the total incidence of complications were lower than those in the traditional group, with statistical significances (P < 0.05). The proportions of grade A and wound healing and painless patients in the laparoscopic group were higher than those in the traditional group, and the proportions of grade B and C, mild discomfort, moderate pain and severe pain were lower than those in the traditional group, with statistical significances (P < 0.05). The levels of hypersensitive C-reactive protein (hs-CRP), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the two groups at 1d and 3d days after operation were higher than those before operation, and the levels of hs-CRP, IL-6 and TNF-α in the laparoscopic group at 1d and 3d days after operation were lower than those in the traditional group, the differences were statistically significant (P < 0.05). Conclusion Single-port laparoscopic high ligation of the vaginal process for hydrocele can optimize the operation, reduce inflammation, relieve pain in children, contribute to postoperative recovery, shorten the hospital stay, and improve the safety of treatment.