肺结核患者外周血 MPT64、IL–12 及 T 淋巴细胞亚群检测及分析

Detection and Analysis of MPT64, IL-12 and T Lymphocyte Subsets in Peripheral Blood of Patients with Pulmonary Tuberculosis

摘要:

〔摘 要〕 目的：分析肺结核（PTB）患者外周血结核分枝杆菌（Mtb）分泌蛋白 MPT64、白细胞介素 –12（IL–12） 及 T 淋巴细胞亚群相关参数的变化。方法：以 2019 年 3 月至 2021 年 2 月深圳市龙岗中心医院收治的 PTB 患者 56 例 为观察组，根据病程分为 PTB 活动期 30 例，稳定期 26 例。另取同期体检健康人 56 例为对照组。酶联免疫吸附法检 测两组研究对象血清 MPT64、IL–12 水平，流式细胞仪检测两组研究对象的外周血 T 淋巴细胞亚群相关指标水平， 并对外周血 MPT64、IL–12 与 T 淋巴细胞亚群参数进行相关性分析。结果：观察组患者血清 MPT64、IL–12 及外周 血 CD3+ 、CD4+ 、CD8+ 水平均高于对照组，而 CD4+ /CD8+ 水平低于对照组，差异均有统计学意义（P ＜ 0.05）。 经 Pearson 相关性分析，PTB 患者外周血 CD4+ 以及 CD8+ 均与 MPT64、IL–12 呈正相关，而 CD4+ /CD8+ 与 MPT64、 IL–12 呈负相关。结论：通过检测 T 淋巴细胞亚群与血清 MPT64、IL–12 变化情况，可为 PTB 的诊断、治疗以及预 后评估提供参考依据。〕 目的：分析肺结核（PTB）患者外周血结核分枝杆菌（Mtb）分泌蛋白 MPT64、白细胞介素 –12（IL–12） 及 T 淋巴细胞亚群相关参数的变化。方法：以 2019 年 3 月至 2021 年 2 月深圳市龙岗中心医院收治的 PTB 患者 56 例 为观察组，根据病程分为 PTB 活动期 30 例，稳定期 26 例。另取同期体检健康人 56 例为对照组。酶联免疫吸附法检 测两组研究对象血清 MPT64、IL–12 水平，流式细胞仪检测两组研究对象的外周血 T 淋巴细胞亚群相关指标水平， 并对外周血 MPT64、IL–12 与 T 淋巴细胞亚群参数进行相关性分析。结果：观察组患者血清 MPT64、IL–12 及外周 血 CD3+ 、CD4+ 、CD8+ 水平均高于对照组，而 CD4+ /CD8+ 水平低于对照组，差异均有统计学意义（P ＜ 0.05）。 经 Pearson 相关性分析，PTB 患者外周血 CD4+ 以及 CD8+ 均与 MPT64、IL–12 呈正相关，而 CD4+ /CD8+ 与 MPT64、 IL–12 呈负相关。结论：通过检测 T 淋巴细胞亚群与血清 MPT64、IL–12 变化情况，可为 PTB 的诊断、治疗以及预 后评估提供参考依据。

Abstract:

〔Abstract〕 Objective To investigate the changes of Mycobacterium tuberculosis (Mtb) secretory protein MPT64, interleukin-12 (IL-12) and T lymphocyte subsets in peripheral blood of patients with pulmonary tuberculosis (PTB). Methods A total of 56 PTB patients admitted to Longgang Central Hospital from March 2019 to February 2021 were selected as the observation group. According to the course of disease, they were divided into 30 cases of active PTB and 26 cases of stable PTB. 56 healthy people in the same period were selected as the control group. The levels of serum MPT64 and IL-12 in the two groups were detected by enzyme-linked immunosorbent assay, and the levels of peripheral blood T lymphocyte subsets related indicators in the two groups were detected by flow cytometry, and the correlation between peripheral blood MPT64, IL-12 and T lymphocyte subsets parameters was analyzed. Results The levels of serum MPT64, IL-12 and peripheral blood CD3+ , CD4+ , CD8+ in the observation group were higher than those in the control group (P < 0.05), while the CD4+ /CD8+ level was lower than that in the control group (P < 0.05). Pearson correlation analysis showed that CD4+ , CD8+ in peripheral blood of PTB patients were positively correlated with MPT64 and IL-12. The ratio of CD4+ /CD8+ was negatively correlated with MPT64 and IL-12. Conclusion By detecting the changes of T lymphocyte subsets and serum MPT64 and IL-12, it can provide reference for the diagnosis, treatment and prognosis evaluation of PTB.