Abstract:〔Abstract〕 Objective To explore the role of pharmaceutical intervention of pharmacists in dispensing prescription in drug pharmacy. Methods A total of 920 dispensing prescriptions in the Joint Logistics Support Force 907 Hospital pharmacy from March 2022 to April 2023 were selected as the study objects and divided into different groups according to different time periods, including March 2022 to September 2022 as a control group (420 cases) and October 2022 to March 2023 as the observation group (500 cases). The control group received routine prescription intervention, and the observation group received prescription dispensing from pharmacists' pharmaceutical intervention pharmacy on the basis of the control group, and the intervention effect was analyzed. Results The incidence of irrational drug use in the observation group was 6.00 %, lower than that in the control group (9.52 %), and the difference was statistically significant (P < 0.05). The observation group was lower than the control group with single drug, and the situation of double drug, triple drug and above was higher, the difference was statistically significant (P < 0.05). The scores of pharmacy staff in the observation group were higher, and the difference, were statistically significant (P < 0.05). Conclusion Pharmacists' pharmaceutical intervention in the prescription dispensing of drug pharmacies can effectively improve the irrational use of drugs, increase the situation of drug combination, improve the professional identity of pharmacy staff, improve their work efficiency and enthusiasm.