长生解毒汤抑制新型冠状病毒复制的体外实验研究

In Vitro Experimental Study on the Inhibition of SARS–CoV–2 Replication by ChangShengJieDu Decoction

摘要:

〔摘 要〕 目的：长生解毒汤是由天然本草及食物精华组成的具有扶正解毒功效的中医方剂，但是否能抑制新型冠状病 毒的复制并不清楚，本研究旨在探讨长生解毒汤对于新型冠状病毒的抑制作用。 方法：建立非洲绿猴肾上皮（Vero）细胞 细胞系，使用不同稀释度的长生解毒汤进行细胞毒性试验以及实时荧光定量核酸扩增（QPCR）检测。 结果：长生解毒汤 在稀释度高于 1:16 时，对 Vero 细胞几乎没有毒性；长生解毒汤在体外可以抑制严重急性呼吸综合征冠状病毒 2（SARS– CoV–2）的增殖，抑制 SARS–CoV–2 拷贝数 50 % 的长生解毒汤的稀释度为 1:45.024。当稀释度为 1:32 时，长生解毒汤对 于细胞毒性最低且对于 SARS–CoV–2 野生株的抑制程度较好。 结论：长生解毒汤对于新型冠状病毒具有一定的抑制作用， 且对 Vero 细胞毒性较低。

关键词:急性呼吸综合征冠状病毒 2;长生解毒汤;新型冠状病毒感染;中药

Abstract:

〔Abstract〕 Objective The purpose of this study was to look into the inhibitory effect of ChangShengJieDu decoction on SARS– CoV–2. ChangShengJieDu decoction is a traditional Chinese medicine formula made up of natural herbs and food extracts that have been shown to support justice and detoxification, but it is unclear whether it can inhibit the replication of SARS–CoV–2. Methods Vero cell line was established, and toxicity test and QPCR detection were performed with different dilutions of ChangShengJieDu decoction. Results ChangShengJieDu decoction had almost no toxicity to Vero cells at dilution higher than 1:16; ChangShengJieDu decoction could inhibit the proliferation of SARS–CoV–2 in vitro, and the dilution of ChangShengJieDu decoction that inhibited 50% of SARS–CoV–2 copies was 1:45.024. When the dilution was 1:32, ChangShengJieDu decoction had the lowest cytotoxicity and the best inhibition of wild SARS–CoV–2. Conclusion ChangShengJieDu decoction has certain inhibitory effect on SARS–CoV–2, and has low toxicity to Vero cells.

Key words:SARS–CoV–2; ChangShengJieDu decoction; COVID-19; Traditional Chinese Medicine