S100 蛋白和 CD43 在涎腺腺样囊性癌和 基底细胞腺瘤中的表达及意义

Expression and Significance of S100 and CD43 in Adenoid Cystic Carcinoma and Basal Cell Adenoma

摘要:

〔摘 要〕 目的：探讨 S100 蛋白和簇分化抗原（CD）43 免疫组化标记在涎腺腺样囊性癌（ACC）和基底细胞腺瘤 （BCA）中的表达及意义。 方法：收集复旦大学附属中山医院厦门医院 2018 年 1 月至 2023 年 12 月间手术切除的 21 例 ACC 和 8 例 BCA 患者病灶标本，采用免疫组化 EnVision 两步法行 S100 蛋白和 CD43 免疫组化染色并分析表达情况。 结果：S100 蛋白标记显示所有 ACC 病例均未见 S100 蛋白阳性的梭形细胞间质（0.0 %），BCA 可见 S100 蛋白强阳 性的梭形细胞间质（62.5 %），差异具有统计学意义（P ＜ 0.01）；CD43 标记显示 ACC 肿瘤细胞 CD43 阳性表达率 为 71.4 %，而 BCA 肿瘤细胞 CD43 阳性表达率为 12.5 %，差异具有统计学意义（P = 0.01）。 结论：联合 S100 蛋白 和 CD43 免疫组化标记有助于涎腺 ACC 和 BCA 的鉴别诊断。

Abstract:

〔Abstract〕 Objective To investigate the expression and significance of S100 and cluster of differentiation (CD) 43 immunohistochemical staining in adenoid cystic carcinoma (ACC) and basal cell adenoma (BCA). Methods Focal specimens of 21 patients with ACC and 8 patients with BCA who were surgically removed from Zhongshan Hospital, Fudan University (Xiamen Branch) from January 2018 to December 2023 were collected. Immunohistochemical EnVision two-step staining of S100 protein and CD43 were performed and their expressions were analyzed. Results S100 protein labeling showed that no S100 protein positive spindle interstitial was found in all ACC cases (0.0 %), while S100 protein strongly positive spindle interstitial was found in BCA cases (62.5 %), the difference was statistically significant (P < 0.01). The CD43 labeling showed that the CD43 positive expression rate of ACC tumor cells was 71.4 %, while that of BCA tumor cells was 12.5 %, the difference was statistically significant (P = 0.01). Conclusion The combination of S100 and CD43 immunohistochemical staining is helpful for the differential diagnosis of ACC and BCA.